| 167 | 7 | 105 |
| 下载次数 | 被引频次 | 阅读次数 |
为了探讨NaCl胁迫对酸枣幼苗光合生理特性的影响,本研究以水培的酸枣幼苗为试材,研究不同浓度NaCl(0、50、100、150、200 mmol/L)处理对酸枣幼苗光合作用和叶绿素荧光特性的影响。结果表明:与对照相比,随着NaCl胁迫天数和浓度的加大,酸枣幼苗叶片的净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、最大荧光(Fm)、PSⅡ原初光能转化效率(Fv/Fm)、光合电子传递速率(ETR)、光化学猝灭系数(qP)均降低,初始荧光(Fo)、非光化学猝灭系数(NPQ)升高、胞间CO2浓度(Ci)先降低后升高,各指标变化幅度由大到小对应的浓度分别是200 mmol/L>150 mmol/L>100mmol/L>50 mmol/L。NaCl胁迫导致酸枣幼苗光合作用下降的原因是导气孔限制转变成非气孔限制,同时PSⅡ反应中心光合机构受损,引起其光能原初捕捉能力和光合电子传递速率下降,最终导致酸枣幼苗光合作用能力的减弱。
Abstract：In order to improve the salt tolerance of jujube and provide reference for reasonable utilization of saline land, the effects of different concentrations of NaCl stress(0, 50, 100, 150 and 200 mmol/L) on photosynthesis and chlorophyll fluorescence characteristics of wild jujube seedlings were investigated in this study. The results showed that compared with the control, with the increase of NaCl concentrations and stress times, the net photosynthetic rate(Pn), net stomatal conductance(Gs), transpiration rate(Tr), maximum fluorescence yield(Fm) and PS II efficiency of primary conversion of light energy(Fv/Fm), photosynthetic electron transport rate(ETR), and photochemical quenching coefficient(q P) decreased; initial fluorescence(Fo), non-photochemical quenching(NPQ) increased; intercellular CO2 concentration(Ci) decreased first and then increased. The decreasing amplitude of each index increased with the increase of concentration, which was 200 mmol/L < 150 mmol/L < 100mmol/L < 50 mmol/L. The photosynthesis of sour jujube under NaCl stress was mainly limited by non-stomatal factors rather than stomatal factors.Furthermore, NaCl stress damaged the PS II reaction center, caused the primary capture capacity and the photosynthetic electron transport rate decrease, resulting in the reduced ability of photosynthesis in sour jujube in the end.
[1]Munns R.Comparative physiology of salt and water stress[J].Plant Cell and Environment,2002,25:239-250.
[2]艾瓦努尔.新疆水资源开发与生态环境保护建设初探[J].干旱环境监测,2001,15(3):1-3.Ai WNE.Water resources development and ecological environment protection in Xinjiang[J].Arid Environmental Monitoring,2001,15(3):130-132.
[3]许鹏,安沙舟.新疆北疆平原荒漠区草地资源与经营系统[J].中国草地,1995(5):3-9.Xu P,An S Z.Grassland resources and management system in the northern Xinjiang Plain Desert Area[J].Grassl China,1995(5):3-9.
[4]胡明芳,田长彦,赵振勇,等.新疆盐碱地成因及改良措施研究进展[J].西北农林科技大学学报(自然科学版),2012,40(10):111-117.Hu M F,Tian C Y,Zhao Z Y,et al.Salinization causes and research progress of technologies improving salinealkali soil in Xinjiang[J].J Northwest A&F Univ(Natural Science),2012,40(10):111-117.
[5]刘孟军,汪民.中国枣种质资源[M].北京中国林业出版社,2009:41-45.
[6]Bethke P C,Drew M C.Stomatal and nonstomatal components to inhibition of photosynthesis in leaves of Capsicum annuum during progressive exposure to Na Cl salinity[J].Plant Physiology,1992,99:219-226.
[7]Wolf D J.Effect of Na Cl salinity on flows and partitioning of C.N.and mineral ions in whole plants of white lupin,Lupinus albus L.[J].J Exp Bot,1992,43(251):777-788.
[8]Munns R,Greenway H,Delane R R,et al.Ion concentration and carbohydrate status of the elongating leaf tissue of Hordeum vulgare growing at high external Na Cl[J].J Exp Bot,1982,33(135):574-583.
[9]Sultana N,Ikeda T,Itoh R.Effect of Na Cl salinity on phoosynthesis and dry matter accumulation in developing rice grains[J].Environ Exp Bot,1999,42:211-220.
[10]Zhu X G,Zhang Q D.Advances in the research on the effects of Na Cl on photosynthesis[J].Chin Bull Bot,1999,16(4):332-338.
[11]Seemann J R,Sharkey T D.Salinity and nitrogen effects on photosynthesis,ribulose-1,5-bisphosphate carboxylase and metabolite pool sizes in Phaseolus vulgaris L.[J].Plant Physiol,1986,82:555-560.
[12]Meyer S,Kouchkovsky Y.Electron transport,photosystem-2reaction centers and chlorophyll protein complexes of thylakoids of drought resistant and sensitive lupin plants[J].Photosynth Res,1993,37:49-60.
[13]Brugnoli E,Lauteri M.Effects of salinity on stomatal conductance,photosynthetic capacity,and carbon isotope discrimination of salt tolerant(Gossypium hirsutum L.)and salt sensitive(Phaseolus vulgaris L.)C3 non-halophytes[J].Plant Physiol,1991,95:628-635.
[14]Satoh K,Smith C M,Fork D C.Effect of salinity on primary processes of photosynthesis in the red alga porphyraperforata[J].Plant Physiol,1983,73:643-647.
[15]Baker N R.A possible role for photosystemⅡin environmental perturbations of photosynthesis[J].Physiologia Plantarum,1991,81(4):563-570.
[16]李秀霞,齐曼·尤努斯,高桥久光,等.Na2SO4胁迫对沙枣光合速率及其生理指标的影响[J].新疆农业科学,2005,42(2):102-106.Li X X,Qiman.Y N S,Gao Q J G,et al.Effects of Na2SO4stress on net photosynthesis rate and other physiological indexes of elaeagnus mooraroftii[J].Xinjiang Agricultural Sciences,2005,42(2):102-106.
[17]高光林,姜卫兵,汪良驹.等.砧木对盐处理下‘丰水’梨幼树光合特性的影响[J].园艺学报,2003,30(3):258-262.Gao G L,Jiang W B,Wang L J,et al.Effects of rootstocks on photosynthetic properties of Young-Hosui[J].Pear trees under Salinity Acta Hortic Sin[J].2003,30(3):258-262.
[18]Water R R,Blackmore D H,Sun Q.Carbon dioxide assimilation and foliar ion concentration in leaves of lemon(Citrus limon L.)trees irrigated with Na Cl or Na2SO4[J].J Plant Physiol,1993,20:173-185
[19]Schreiner M,Ludders P.Gas exchange of apple trees under salinity and levels of K+supply[J].Gartenbauwissenscha,1996,6(3):130-138.
[20]Iqbal M,Ashraf M.Changes in growth,photosynthetic capacity and ionic relation in spring wheat(Triticum aestivum L.)due to presowing seed treatment with polyamines[J].Plant Growth Regulation,2005,46(1):19-30.
[21]王策,鲁晓燕,樊新民,等.Ca Cl2对Na Cl胁迫下酸枣幼苗抗氧化酶活性和膜脂过氧化作用的影响[J].石河子大学学报(自然科学版),2014,32(3):285-290.Wang C,Lu X Y,Fan X M,et al.Effects of Ca Cl2on antioxidant enzyme activity and lipid peroxidant of sour jujube seedlings under Na Cl stress[J].Journal of Shihezi University(Natural Science),2014,32(3):285-290.
[22]靳娟,王依,鲁晓燕,等.Na Cl胁迫对酸枣幼苗离子吸收与分配的影响[J].园艺学报,2015,42(5):853-862.Jin J,Wang Y,Lu X Y,et al.Effects of Na Cl stress on ion absorption and distribution in sour jujube seedlings[J].Acta Hortic Sin,2015,42(5):853-862.
[23]吕新民,杨一帆,鲁晓燕,等.Na Cl胁迫对酸枣幼苗As AGSH循环的影响[J].植物生理学报,2016,52(5):736-744.Lv X M,Yang Y F,Lu X Y,et al.Effects of Na Cl stress on the As A–GSH cycle in sour jujube seedlings[J].Plant Physiol J,2016,52(5):736-744.
[24]郭世荣.无土栽培学[M].北京:中国农业出版社,2003:87-90.
[25]惠红霞,许兴,李前荣.外源甜菜碱对盐胁迫下枸杞光合功能的改善[J].西北植物学报,2003,23(12):2137-2422.Hui H X,Xu X,Li Q R.Exogenous betaine improves photosynthesis of Lycium barbarumunder salt stress[J].Acta Bot,Bor-Occid.Sin.2003,23(12):2137-2422.
[26]Kurban H,Saneoka H,Nehira K,et al.Effect of salinity on growth,photosynthesis and mineral composition in leguminous plant A.lhagi pseudoalhagii(Bieh.)[J].Soil Sci Plant Nutr,1999(45):851-862.
[27]金雅琴,李冬林,丁雨龙,等.盐胁迫对乌桕幼苗光合特性和叶绿素含量的影响[J].南京林业大学学报(自然科学版),2011,35(1):29-33.Jin Y Q,Li D L,Ding Y L,et al.Effects of salt stress on photosynthetic characteristics and chlorophyll content of Sapium sebiferum seedlings[J].Journal of Nanjing Forestry University(Natural Science Edition),2011,35(1):29-33.
[28]陈健妙,郑青松,刘兆普,等.麻疯树幼苗生长和光合作用对盐胁迫的响应[J].生态学报,2009,29(3):1356-1365.Chen J M,Zheng Q S,Liu Z P,et al.Growing and photosynthetic response of Jatropha curcas L.seedlings to salt stress[J].Acta Ecologic Sic,2009,29(3):1356-1365.
[29]Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annu Rev Plant Physio1,1982,33:317-345.
[30]李宏,邓江宇,张振春.盐胁迫对盐桦幼苗光合特性的影响[J].新疆农业科学,2010,47(2):213-217.Li H,Deng J H,Zhang Z C.Influence of salt stress on physiological adaptability of young Betula halophila trees[J].Xinjiang Agricultural Sci,2010,47(2):213-217.
[31]Lu C M,Zhang J H.Effects of water stress on photosystemⅡphotochemistry and its thermostability in wheat plants[J].Journal of Experimental Botany,1999,50(336):1199-1206.
[32]张璐颖,文笑,林勇明,等.盐胁迫对台湾恺木幼苗光合作用和荧光特性的影响[J].福建林学院学报,2013,33(3):193-199.Zhang L Y,Wen Xiao,Lin Y M,et al.Effect of salt stress on photosynthetic and chlorophyll fluorescent characteristics in Alnus formosana seedlings[J].Journal of Fujian College of Forestry,2013,33(3):193-199.
[33]徐凯,郭延平,张上隆.草莓叶片光合作用对强光的响应及其机理研究.应用生态学报,2005,16(1):73-78.Xu K,Guo Y P,Zhang S L.Response of strawberry leaves photosynthesis to strong light and its mechanism[J].Chinese Journal of Applied Eco,2005,16(1):73-78.
基本信息:
DOI：10.13880/j.cnki.65-1174/n.2017.03.009
中图分类号:S665.1
引用信息:
[1]赵娟娟,孟瑛,朱海峰等.NaCl胁迫对酸枣(Ziziphus acidojujuba)幼苗光合作用和荧光特性的影响[J],2017,35(03):319-325.DOI:10.13880/j.cnki.65-1174/n.2017.03.009.
基金信息:
国家自然科学基金项目(31460057)